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A Broad-Spectrum Inhibitor of CRISPR-Cas9

Highlights •Phage-encoded anti-CRISPRs (Acrs) inhibit CRISPR-Cas9 through unique mechanisms •AcrIIC1 controls genome editing by diverse Cas9 orthologs •AcrIIC1 binding disables Cas9’s nucleases without impacting DNA binding •AcrIIC3 promotes dimerization of Cas9 and prevents DNA...

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CRISPR Corrects RNA-based Disease Defects

Armed with a modified version of CRISPR-Cas9, scientists have removed nucleotide repeats from RNA in vitro that are known to cause diseases, namely, Huntington’s, ALS, and myotonic dystrophy. Cells from patients with myotonic dystrophy...

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Tiny Answers to Big Questions

The most abundant biological entities on Earth still have a few tricks up their sleeve, and a recent surge of research is reinvigorating the field. With the potential to dramatically expand known protein sequence...

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CRISPR Corrects Duchenne-Causing Mutations

Researchers have been studying the CRISPR-Cas9 gene-editing system as a potential therapeutic tool to modify or delete pathogenic sequences within the human genome. Now, using a more recently discovered system, CRISPR-Cpf1, researchers have demonstrated...

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Rethinking a Cancer Drug Target

A protein thought to be essential for cancer cells may not be so essential after all, according to a study published today (March 24) in eLife. Researchers at Cold Spring Harbor Laboratory (CSHL) in...

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The Birth of CRISPR Inc

In early 2012, Emmanuelle Charpentier, a little-known French microbiologist who would soon meet worldwide fame, contacted her old friend Rodger Novak to tell him about her recent studies at Umeå University in Sweden of...

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New CRISPR-Cas Enzymes Discovered

Microbes use a number of CRISPR-Cas systems for immunity, but it’s the class 2 system, typically utilizing the nuclease Cas9, that has been adapted for genome editing. A metagenomics search of microbes sampled from...

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Life Science Controversies of 2016

Two ongoing disputes brought The Scientist to the courtroom to listen in on the proceedings this year. The first is a defamation suit that former Wayne State University researcher Fazlul Sarkar brought against anonymous...

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Human CRISPR Trials Could Become Sputnik 2.0

It was the injection heard around the world. On October 28th, a team of scientists at Sichuan University in Chengdu, China, delivered the first cells that had been genetically altered using the revolutionary genome-editing...

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Harvest time for CRISPR-Cas?

The revolutionary gene editing technology CRISPR-Cas is on the verge of widespread application in agriculture, promising easy access to dramatic crop improvements. Companies now licensing the technology face the challenge of regaining the public...

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Targeting the Noncoding Genome with CRISPR

Demonstrating yet another basic research application of CRISPR, two teams have independently reported scaled up CRISPR interference (CRISPRi) and CRISPR/Cas9 noncoding genomic screens. Researchers at MIT and the Broad Institute of MIT and Harvard used...

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CRISPR: No Cutting Required

Taking advantage of a deaminase enzyme that introduces a single nucleotide change to DNA, researchers have created a modified CRISPR/Cas9tool that avoids the generation of a deleterious double-stranded break, minimizes the potential for the...

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A New CRISPR System for RNA

While the CRISPR/Cas9 genome editing system has been “burning up the charts”, so to speak, with its extraordinary versatility and potential for treating a host of diseases, until now its editing capabilities have been...

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